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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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A simple gas-liquid chromatographic method with electron capture detection for the determination of progesterone in the blood of humans and domestic animals.

A simple routine method is reported for the determination of progesterone in blood by gas-liquid chromatography. An unlabelled internal standard, testosterone acetate, was added to plasma samples. Following preliminary purification by thin-layer chromatography, progesterone in pregnancy plasma was evaluated as the free steroid with a flame ionization detector. The hormone in the plasma of women during the menstrual cycle and of cycling domestic animals was determined as the 3-enol ester heptafluorobutyrate by electron capture detection. The validity of testosterone acetate as an internal standard was proved by simultaneous processing of [7alpha-3H]progesterone and a [4-14C]testosterone acetate and the determination of the isotope ratio in samples. The results of control experiments and normal values are also presented.[1]

References

  1. A simple gas-liquid chromatographic method with electron capture detection for the determination of progesterone in the blood of humans and domestic animals. Fehér, T., Fehér, K.G., Bodrogi, L. J. Chromatogr. (1975) [Pubmed]
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