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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Cloning, high-level expression, purification and crystallization of peptide deformylase from Leptospira interrogans.

A new peptide deformylase ( PDF; EC 3.5.1.27) gene from Leptospira interrogans was identified and cloned into expression plasmid pET22b(+) and was highly expressed in Escherichia coli BL21(DE3). With DEAE-Sepharose anion-exchange chromatography followed by Superdex G-75 size-exclusion chromatography, 60 mg of PDF from L. interrogans was purified from 1 l of cell culture. Crystallization screening of the purified enzyme resulted in two crystal forms, from one of which a 3 A resolution X-ray diffraction data set has been collected.[1]

References

  1. Cloning, high-level expression, purification and crystallization of peptide deformylase from Leptospira interrogans. Li, Y., Ren, S., Gong, W. Acta Crystallogr. D Biol. Crystallogr. (2002) [Pubmed]
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