Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Shetzline, S.E. et al.

Structural and functional features of the 37-kDa 2-5A-dependent RNase L in chronic fatigue syndrome.

A 2',5'-oligoadenylate (2-5A)-dependent 37-kDa form of RNase L has been reported in extracts of peripheral blood mononuclear cells (PBMC) from individuals with chronic fatigue syndrome (CFS). In the current study, analytic gel permeation FPLC, azido photoaffinity labeling, two-dimensional (2-D) gel electrophoresis, and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) have been used to examine the biochemical relationship between the 80-kDa RNase L in healthy control PBMC and the 37-kDa RNase L in PBMC from individuals with CFS. Like the 80-kDa RNase L, the 37-kDa RNase L is present as a catalytically inactive heterodimer complex with the RNase L inhibitor (RLI). Formation of a 37-kDa RNase L-RLI complex indicates that the 37-kDa RNase L is structurally similar to the 80-kDa RNase L at the N-terminus, which contains the 2-5A binding domain. The enzymatically active monomer form of 37-kDa RNase L resolved by 2-D gel electrophoresis has a pI of 6. 1. RT-PCR and Southern blot analyses demonstrated that the 37-kDa RNase L is not formed by alternative splicing. In-gel tryptic digestion of the 37-kDa RNase L that was excised from 2-D gels and subsequent MALDI-MS analysis identified three peptide masses that are identical to three predicted peptide masses in the 80-kDa RNase L. The electrophoretic mobility of 2-5A azido photolabeled/immunoprecipitated 37-kDa RNase L was the same under reducing and nonreducing conditions. The results presented show that the 37-kDa form of RNase L in PBMC shares structural and functional features with the native 80-kDa RNase L, in particular in the 2-5A binding and catalytic domains.[1]

References

Structural and functional features of the 37-kDa 2-5A-dependent RNase L in chronic fatigue syndrome. Shetzline, S.E., Martinand-Mari, C., Reichenbach, N.L., Buletic, Z., Lebleu, B., Pfleiderer, W., Charubala, R., De Meirleir, K., De Becker, P., Peterson, D.L., Herst, C.V., Englebienne, P., Suhadolnik, R.J. J. Interferon Cytokine Res. (2002) [Pubmed]

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