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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Quantitative chromatographic method for the determination of low levels of nitrilotriacetic and ethylenediaminetetraacetic acids in diethylenetriaminepentaacetic acid.

A quantitative method for determination of low levels (0.05%, w/w) of nitrilotriacetic and ethylenediaminetetraacetic acids in diethylenetriaminepentaacetic acid is described. Palmitic acid is added to the chelator as an internal standard before esterification with methanol containing 2%(v/v)H2SO4. The methyl esters of palmitic, nitrilotriacetic, and ethylenediaminetetraacetic acids are first separated from diethylenetriaminepentaacetate by silicic acid column chromatography and are subsequently quantitated by gas-liquid chromatography. The method is both accurate and reproducible with less than 10% relative error. Thin-layer chromatographic separations of the methyl esters, and quantitation at the 1% level, are also described.[1]

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