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McVicar, D.W. et al.

McVicar, Winkler-Pickett, Taylor, Makrigiannis, Bennett, Anderson, Ortaldo,

Aberrant DAP12 signaling in the 129 strain of mice: implications for the analysis of gene-targeted mice.

NK cells are implicated in antiviral responses, bone marrow transplantation and tumor immunosurveillance. Their function is controlled, in part, through the Ly49 family of class I binding receptors. Inhibitory Ly49s suppress signaling, while activating Ly49s (i.e., Ly49D) activate NK cells via the DAP12 signaling chain. Activating Ly49 signaling has been studied primarily in C57BL/6 mice, however, 129 substrains are commonly used in gene-targeting experiments. In this study, we show that in contrast to C57BL/6 NK cells, cross-linking of DAP12- coupled receptors in 129/J mice induces phosphorylation of DAP12 but not calcium mobilization or cytokine production. Consistent with poor-activating Ly49 function, 129/J mice reject bone marrow less efficiently than C57BL/6 mice. Sequence analysis of receptors and DAP12 suggests no structural basis for inactivity, and both the 129/J and C57BL/6 receptors demonstrate normal function in a reconstituted receptor system. Most importantly, reconstitution of Ly49D in 129/J NK cells demonstrated that the signaling deficit is within the NK cells themselves. These unexpected findings bring into question any NK analysis of 129/J, 129Sv, or gene-targeted mice derived from these strains before complete backcrossing, and provide a possible explanation for the differences observed in the immune response of 129 mice in a variety of models.[1]

References

Aberrant DAP12 signaling in the 129 strain of mice: implications for the analysis of gene-targeted mice. McVicar, D.W., Winkler-Pickett, R., Taylor, L.S., Makrigiannis, A., Bennett, M., Anderson, S.K., Ortaldo, J.R. J. Immunol. (2002) [Pubmed]

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