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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Functional mapping of Saccharomyces cerevisiae Prp45 identifies the SNW domain as essential for viability.

The essential gene product Prp45 (379 aa) of Saccharomyces cerevisiae is a highly conserved, but N-terminally abridged, ortholog of the human transcriptional coactivator SKIP, which is involved in TGFbeta, Notch, and steroid hormone signaling. We used a diploid strain harboring PRP45 deletion, which is inviable in the haploid, to test for complementation with the truncated versions of Prp45. The N-terminal half of the protein (aa 1 to 190), denoted as the SNW domain, was found sufficient to support the essential function. Interestingly, substituting the SNW motif itself with AAA was compatible with viability. GFP-tagged Prp45 was localized in nuclear "speckles" over a diffuse nuclear background. We further found that Prp45 activated the transcription of a reporter gene in S. cerevisiae when targeted to DNA. The observed effect relied in part upon the presence of conserved helical repeats and upon the highly charged C-terminal domain (pI = 11.3). Prp45, which lacks most of the binding motifs of the human ortholog, and whose N-terminal half is sufficient for supporting the growth of prp45 cells, might be helpful in elucidating the essential function of SNW/SKIP proteins.[1]


  1. Functional mapping of Saccharomyces cerevisiae Prp45 identifies the SNW domain as essential for viability. Martinkova, K., Lebduska, P., Skruzny, M., Folk, P., Puta, F. J. Biochem. (2002) [Pubmed]
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