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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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A novel active DNA topoisomerase I in Leishmania donovani.

A common feature shared by type I DNA topoisomerases is the presence of a "serine, lysine, X, X, tyrosine" motif as conventional enzyme active site. Preliminary data have shown that Leishmania donovani DNA topoisomerase I gene (LdTOP1A) lacked this conserved motif, giving rise to different theories about the reconstitution of an active DNA topoisomerase I in this parasite. We, herein, describe the molecular cloning of a new DNA topoisomerase I gene from L. donovani (LdTOP1B) containing the highly conserved serine, lysine, X, X, tyrosine motif. DNA topoisomerase I activity was detected only when both genes (LdTOP1A and LdTOP1B) were co-expressed in a yeast expression system, suggesting the existence of a dimeric DNA topoisomerase I in Leishmania parasites.[1]

References

  1. A novel active DNA topoisomerase I in Leishmania donovani. Villa, H., Otero Marcos, A.R., Reguera, R.M., Balaña-Fouce, R., García-Estrada, C., Pérez-Pertejo, Y., Tekwani, B.L., Myler, P.J., Stuart, K.D., Bjornsti, M.A., Ordóñez, D. J. Biol. Chem. (2003) [Pubmed]
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