Stimulation of growth by both androgen and estrogen of the EMP- K1 transplantable tumor with androgen and estrogen receptors from human extramammary Paget's disease in nude mice.
BACKGROUND: Experimental approaches using transplantable human tumors in nude mice have greatly improved studies on their pathogenesis and treatment. PURPOSE: We studied the establishment of a transplantable tumor, EMP- K1, derived from extramammary Paget's disease and the morphology and unique hormone dependence of this tumor. METHODS: Tissue fragments from a metastatic skin tumor of an 80-year-old man with primary genital extramammary Paget's disease were implanted into male nude BALB/c mice. Tissue fragments of the established tumor were implanted into 50 castrated male 8-week-old nude mice, which were given injections of 100 micrograms testosterone propionate (TP), 100 micrograms 5 alpha-dihydrotestosterone (DHT), 10 micrograms diethylstilbestrol (DES), or 1, 10, or 100 micrograms 17 beta-estradiol (E2). All injections were administered intramuscularly once daily, starting from the day of transplantation. The established tumors were examined immunohistochemically and biochemically. RESULTS: A transplantable tumor ( EMP- K1) was established in the nude mouse. EMP- K1 tumor cells expressed antigens such as carcinoembryonic antigen and epithelial membrane antigen, cytokeratin, and c-erbB-2 protein and contained androgen, estrogen, and progesterone receptors. The growth of the EMP- K1 tumor was stimulated by TP, DHT, DES, and E2. CONCLUSIONS: These results suggest that both androgen and estrogen may stimulate the growth of the same tumor by both androgen receptor and estrogen receptor pathways. IMPLICATIONS: The EMP- K1 tumor is a useful tool for studies on the biology of extramammary Paget's disease, and further studies using these tumors will provide useful information concerning proper hormone therapy.[1]References
- Stimulation of growth by both androgen and estrogen of the EMP-K1 transplantable tumor with androgen and estrogen receptors from human extramammary Paget's disease in nude mice. Nishi, M., Tashiro, M., Yoshida, H. J. Natl. Cancer Inst. (1992) [Pubmed]
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