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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Colocalization of somatostatin receptors and growth hormone-releasing factor immunoreactivity in neurons of the rat arcuate nucleus.

Recent studies from our group have demonstrated an association of [125I]-labeled somatostatin (SRIF)-binding sites with a subpopulation of arcuate (ARC) neurons. The distribution of these cells was similar to that of growth hormone-releasing factor (GRF)-immunoreactive neurons, which led us to propose that at least some SRIF receptors may be directly localized to GRF-containing cells. To test this hypothesis, we have visualized radiolabeled SRIF- binding sites and GRF immunoreactivity (ir) in adjacent sections of the hypothalamus, by combined radioautography and immunohistochemistry. Adult male rats were sacrificed by decapitation and the brains were rapidly frozen and serially sectioned on a cryostat. Fifteen pairs of adjacent 6-microns-thick sections, taken at 100-microns intervals through the rostrocaudal extent of the ARC nucleus, were alternately processed for [125I]-SRIF radioautography and GRF immunohistochemistry. GRF-ir and [125I]-SRIF-labeled cells were mapped at each level and quantified with the aid of a camera lucida. The maps were subsequently superimposed to determine the extent of [125I]-SRIF/GRF-ir colocalization. GRF-ir perikarya [13.2 +/- 4.4 (mean +/- SE) cells per section] were mainly localized in the ventrolateral portion of the ARC nucleus and predominated within the caudal-most tier. [125I]-SRIF-labeled cells (35.6 +/- 6.5 cells per section) were more numerous, more evenly distributed, and extended further rostrally and caudally than GRF-ir cells. Superimposition of the camera lucida maps indicated that, overall, 33.5 +/- 10.8% of the GRF-ir cells were labeled with [125I]-SRIF in adjacent sections.(ABSTRACT TRUNCATED AT 250 WORDS)[1]


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