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Molecular cloning and characterization of SGT1.2, a novel splice variant of Homo sapiens SGT1.

SGT1.2, a novel splice variant of Homo sapiens SGT1 was isolated from a human fetal brain cDNA library. This cDNA is 1404 bp and contains an open reading frame from 68 to 1165 encoding a putative protein of 365 amino acids (SGT1.2) that share 90% identities to Homo sapiens SGT1, suppressor of G2 allele of SKP1 at protein level. RPS-BLAST searching revealed that SGT1.2 have a TPR domain, a p23 domain, a SGS domain and a CS domain. According to the search of the human genome database, SGT1.2 was mapped to human chromosome 13q14.13. Reversed transcription-polymerase chain reaction analysis indicated that it widely expressed in human adult tissues.[1]

References

  1. Molecular cloning and characterization of SGT1.2, a novel splice variant of Homo sapiens SGT1. Zou, X., Ji, C., Wang, L., Wu, M., Zheng, H., Xu, J., Jin, F., Gu, S., Ying, K., Xie, Y., Mao, Y. DNA Seq. (2004) [Pubmed]
 
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