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Barandon, L. et al.

Barandon, Couffinhal, Dufourcq, Alzieu, Daret, Deville, Duplàa,

Repair of myocardial infarction by epicardial deposition of bone-marrow-cell-coated muscle patch in a murine model.

BACKGROUND: Myocardial infarction results in irreversible myocyte loss. In a murine model, we tested the feasibility of a novel repair technique combining bone marrow cell (BMC) transplantation and cardiomyoplasty. METHODS: Myocardial infarction was induced cryogenically in backcrossed ROSA 26 transgenic x C57BL/6J mice (n = 75). Thirty days later, surviving mice (n = 69) were randomized to sham treatment (rethoracotomy only; n = 11), patch only treatment (n = 29), or patch + BMC treatment (n = 29). Abdominal muscle patches were harvested from donor littermates not expressing the beta-galactosidase reporter gene and sutured on the epicardium directly above the infarct zone. Patch only-treated mice received uncoated patches. Patch + BMC-treated mice received patches coated with 5 x 10(6) beta-galactosidase-expressing BMCs embedded in a collagen-rich three-dimensional matrix. RESULTS: Mortality rate was 52% after muscle patch implantation. Bone marrow cells were able to migrate from muscle patch into the infarct zone, as demonstrated by beta-galactosidase immunostaining, and ultimately constituted 8% of all cells in scar tissue (mean +/- standard deviation, 219 +/- 111/mm2). Angiogenesis and cell survival in the scar were improved by patch + BMC treatment. Left ventricular geometry and cardiac function were improved by patch treatment, with or without BMC, although the effects were stronger after patch + BMC treatment. CONCLUSIONS: Epicardial deposition of a BMC-coated muscle patch is a promising approach to restoring cardiac function after myocardial infarction.[1]

References

Repair of myocardial infarction by epicardial deposition of bone-marrow-cell-coated muscle patch in a murine model. Barandon, L., Couffinhal, T., Dufourcq, P., Alzieu, P., Daret, D., Deville, C., Duplàa, C. Ann. Thorac. Surg. (2004) [Pubmed]

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