Coordinated changes in classes of ribosomal protein gene expression is associated with light-induced retinal degeneration.
PURPOSE: To identify genes with altered expression levels in the degenerating retina in a light-induced retinal degeneration (LIRD) model. METHODS: Adult Sprague-Dawley rats were exposed to intense green light for 4 hours. After this treatment, the retinas were excised, RNA was extracted, and a cDNA library was prepared. The cDNA library was differentially cross-screened with probes representing 0-hour and 4-hour light-exposed rat retina. Transcripts with altered expression levels were sequenced and expression was confirmed by Northern blot analysis. Gene-specific primers were designed and used to examine the expression levels of other genes involved in protein synthesis. Promoter sequences of the ribosomal-binding protein (Rbp) genes were analyzed for transcription-binding sites. RESULTS: Of the 10,000 clones that were initially screened, 41 exhibited altered expression levels. Six of these corresponded to five known Rbp genes. Six additional Rbp genes were also examined. In total, 9 of 11 Rbp genes exhibited an increase in expression levels in response to a 4-hour light exposure. In contrast, the transcript levels of elongation factor 1alpha1 and 18S rRNA did not increase. The most abundant transcription factor-binding sites conserved in the promoter regions of all Rbp genes examined in this study include AP-1, Oct-1, V-myb, USF, Pax-4, and the FOX family of transcription factors. CONCLUSIONS: The results indicate that light-induced retinal degeneration (LIRD) is associated with increased expression of specific Rbp genes. These Rbp genes may be involved in mediating visual cell loss in LIRD through a translational or an extraribosomal mechanism.[1]References
- Coordinated changes in classes of ribosomal protein gene expression is associated with light-induced retinal degeneration. Grewal, R., Stepczynski, J., Kelln, R., Erickson, T., Darrow, R., Barsalou, L., Patterson, M., Organisciak, D.T., Wong, P. Invest. Ophthalmol. Vis. Sci. (2004) [Pubmed]
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