Rapid generation of directed and unmarked deletions in Xanthomonas.
We have devised a rapid four-step procedure for the generation of directed and unmarked chromosomal deletions in bacteria, based on the use of a novel cloning vector containing the Bacillus subtilis sacB gene that encodes levansucrase and confers sucrose sensitivity, which can be used for counter-selection. Using this technique, we describe the construction of a 6.5 kb directed and unmarked deletion in a phytopathogenicity region of the chromosome in Xanthomonas campestris. This procedure allows rapid and easy transfer of a wide variety of mutant allelic DNA to the bacterial chromosome, and should be adaptable to various bacteria besides Xanthomonas spp.[1]References
- Rapid generation of directed and unmarked deletions in Xanthomonas. Kamoun, S., Tola, E., Kamdar, H., Kado, C.I. Mol. Microbiol. (1992) [Pubmed]
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