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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Utilising retention correlation for the separation of oligostyrenes by coupled-column liquid chromatography.

The separation of n = 2-5 n-butyloligostyrenes has been illustrated by reversed-phase reversed-phase (RP-RP) coupled-column liquid chromatography. The coupled-column separation has been achieved by use of a C18 column with methanol as the mobile phase followed by a DiamondBond C18 column with acetonitrile (ACN) mobile phase. The DiamondBond C18 is a hybrid carbon clad zirconia (CCZ)-C18 stationary phase. Unlike a C18-carbon clad zirconia two-dimensional liquid chromatographic system, which is orthogonal, the C18 and DiamondBond C18 columns combination exhibit correlations based upon the molecular weight of n-butyloligostyrenes. Using an alternative strategy to two-dimensional liquid chromatography, the molecular weight dependence displayed by both the C18 column and DiamondBond C18 has been used to increase throughput or decrease analysis time in the analysis of the n-butyloligostyrenes. However, this is at the expense of a portion of the two-dimensional peak capacity displayed by the C18-carbon clad zirconia system.[1]


  1. Utilising retention correlation for the separation of oligostyrenes by coupled-column liquid chromatography. Gray, M.J., Dennis, G.R., Slonecker, P.J., Shalliker, R.A. Journal of chromatography. A. (2005) [Pubmed]
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