ZNF328, a novel human zinc-finger protein, suppresses transcriptional activities of SRE and AP-1.
The zinc finger proteins containing the Kruppel-associated box domain (KRAB-ZFPs) are the single largest class of transcription factors in human genome. Many of the KRAB-ZFPs are involved in cardiac development or cardiovascular diseases. Here, we have identified a novel human KRAB zinc finger gene, named ZNF328, from the human fetal heart cDNA library. The complete sequence of ZNF328 cDNA contains a 2376-bp open reading frame (ORF) and encodes a 792 amino acid protein with an N-terminal KRAB domain and classical zinc finger C2H2 motifs in the C-terminus. Northern blot analysis indicates that the protein is expressed in most of the examined human adult and embryonic tissues. ZNF328 is a transcription suppressor when fused to Gal-4 DNA-binding domain and cotransfected with VP-16. Overexpression of ZNF328 in COS-7 cells inhibits the transcriptional activities of SRE and AP-1. Deletion analysis with a series of truncated fusion proteins indicates that the KRAB motif is a basal repression domain when cotransfected with VP-16. Similar results were obtained when the truncated fusion proteins were assayed for the transcriptional activities of SRE and AP-1. These results suggest that ZNF328 protein may act as a transcriptional repressor in mitogen-activated protein kinase ( MAPK) signaling pathway to mediate cellular functions.[1]References
- ZNF328, a novel human zinc-finger protein, suppresses transcriptional activities of SRE and AP-1. Ou, Y., Wang, S., Cai, Z., Wang, Y., Wang, C., Li, Y., Li, F., Yuan, W., Liu, B., Wu, X., Liu, M. Biochem. Biophys. Res. Commun. (2005) [Pubmed]
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