The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Ligand-mimetic anti-alphaIIb beta3 antibody PAC-1 inhibits tyrosine signaling, proliferation and lung colonization of melanoma cells.

Beta3 integrin expression is the hallmark of melanoma and may serve as a potential therapeutic target. While alphav beta3 integrin expression seems to be constitutive in melanoma, ectopic expression of platelet-alphaIIb beta3 is dependent on progression. B16a murine melanoma is a suitable model for studies on alphaIIb beta3 treatment strategies since alphav beta3 is not expressed in this cell line. Here we have used a ligand-mimetic anti-alphaIIb beta3 monoclonal antibody, PAC-1, to test the biological consequences of alphaIIb beta3 modulation in melanoma cells. We have previously reported that in B16a cells FAK is constitutively active and tyrosine-phosphorylated. Upon PAC-1 binding to the surface alphaIIb beta3, which is in the active conformation, FAK became dephosphorylated through a process of PKC-dependent phosphatase activation. Furthermore, PAC-1 binding to B16a cells induced a significant decrease in phosphotyrosine-positive melanoma cells within 30 min. Treatment of B16a cells in vitro with PAC-1 significantly inhibited proliferation by decreasing the mitotic index but not affecting apoptotic rate. Incubation of B16a cells with PAC-1 decreased their lung colonization potential, suggesting a profound alteration in their biological behavior under the effect of this antibody. These preclinical data suggest that the ectopic expression of alphaIIb beta3 in melanoma cells can be exploited as a novel target of antibody therapy of melanoma.[1]

References

  1. Ligand-mimetic anti-alphaIIb beta3 antibody PAC-1 inhibits tyrosine signaling, proliferation and lung colonization of melanoma cells. Rásó, E., Tóvári, J., Ladányi, A., Varga, N., Tímár, J. Pathol. Oncol. Res. (2005) [Pubmed]
 
WikiGenes - Universities