Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Caminschi, I. et al.

Caminschi, Corbett, Zahra, Lahoud, Lucas, Sofi, Vremec, Gramberg, Pöhlmann, Curtis, Handman, van Dommelen, Fleming, Degli-Esposti, Shortman, Wright,

Functional comparison of mouse CIRE/mouse DC-SIGN and human DC-SIGN.

CIRE/mDC-SIGN is a C-type lectin we originally identified as a molecule differentially expressed by mouse dendritic cell (DC) populations. Immunostaining with a CIRE/mDC-SIGN-specific mAb revealed that CIRE/mDC-SIGN is indeed on the surface of some CD4(+), CD4(-)8(-) DCs and plasmacytoid pre-DCs, but not on CD8(+) DCs. It has been proposed that CIRE/mDC-SIGN is the functional orthologue of human DC-SIGN (hDC-SIGN), a molecule that both enhances T cell responses and facilitates antigen uptake. We assessed if CIRE/mDC-SIGN and hDC-SIGN exhibit functional similarities. CIRE/mDC-SIGN is down-regulated upon activation, but unlike hDC-SIGN, incubation with IL-4 and IL-13 did not enhance CIRE/mDC-SIGN expression, indicating differences in gene regulation. Like hDC-SIGN, CIRE/mDC-SIGN bound mannosylated residues. However, we could detect no role for CIRE/mDC-SIGN in T cell-DC interactions and the protein did not bind to pathogens known to interact with hDC-SIGN, including Leishmania mexicana, cytomegalovirus, HIV and lentiviral particles bearing the Ebolavirus glycoprotein. The binding of CIRE/mDC-SIGN to hDC-SIGN ligands was not rescued when CIRE/mDC-SIGN was engineered to express the stalk region of hDC-SIGN. We conclude that there are significant differences in the fine specificity of the C-type lectin domains of hDC-SIGN and CIRE/mDC-SIGN and that these two molecules may not be functional orthologues.[1]

References

Functional comparison of mouse CIRE/mouse DC-SIGN and human DC-SIGN. Caminschi, I., Corbett, A.J., Zahra, C., Lahoud, M., Lucas, K.M., Sofi, M., Vremec, D., Gramberg, T., Pöhlmann, S., Curtis, J., Handman, E., van Dommelen, S.L., Fleming, P., Degli-Esposti, M.A., Shortman, K., Wright, M.D. Int. Immunol. (2006) [Pubmed]

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