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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Cloning and characterization of a novel soybean receptor-like protein kinase gene GmRLCK.

Protein kinases play key roles in transduction of both environmental and developmental signals in higher plants. Here reported full-length cDNA cloning and preliminary structural and functional analysis of a novel soybean receptor-like protein kinase ( RLK) gene (GenBank Accession No.AY687390). The deduced protein product of this gene contains a transmembrane region, an intracellular catalytic domain possessing serine/threonine kinase activity, and an extracellular domain which lacks the N-terminus signal peptide. Bioinformatic analyses revealed highly similarity between this soybean gene and a group of the Arabidopsis RLK genes which all lack of N-terminus signal sequence and belong to RLCK subfamily. Thus, it was designated GmRLCK. Some residues of GmRLCK that showed high probability to be phosphorylated were also predicted. RT-PCR analysis revealed higher expression levels of GmRLCK in soybean cotyledons, roots, flowers and young pods; whereas lower expression levels were detected in radicles, stems and mature leaves at vegetative stage. Phylogeny analysis suggested that GmRLCK was more close to and might have a common ancestor with several senescence-associated RLKs.[1]

References

  1. Cloning and characterization of a novel soybean receptor-like protein kinase gene GmRLCK. Li, P.L., Ma, Y.Y., Gan, R., Zhang, L.W., Li, X.P., Wang, Y., Wang, N.N. Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology / Zhongguo xi bao sheng wu xue xue hui zhu ban. (2006) [Pubmed]
 
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