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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

THE M AND N GENES-BASED SIMPLEX AND MULTIPLEX PCRs ARE BETTER THAN THE F OR H GENE-BASED SIMPLEX PCR FOR PESTEDES- PETITS-RUMINANTS VIRUS.

Summary. Nucleocapsid ( N), matrix ( M) and hemagglutinin (H) genes-based simplex PCRs and an N and M genes-based multiplex PCR were developed for detection of Peste-des-petits-ruminants virus (PPRV). The M gene PCR was the most sensitive, followed by N, H and an already described fusion (F) gene PCRs, as they could detect the virus in samples with titers of 101, 102, 104and 105 TCID50/ml, respectively. The multiplex PCR was as sensitive as the M gene PCR, but it had the advantage of differentiating PPRV from Rinderpest virus (RPV). Key words: Peste-des-petits-ruminants virus; PCR; RT-PCR; M gene; N gene; H gene; F gene; multiplex PCR.[1]

References

  1. THE M AND N GENES-BASED SIMPLEX AND MULTIPLEX PCRs ARE BETTER THAN THE F OR H GENE-BASED SIMPLEX PCR FOR PESTEDES- PETITS-RUMINANTS VIRUS. George, A., Dhar, P., Sreenivasa, B.P., Singh, R.P., Bandyopadhyay, S.K. Acta Virol. (2006) [Pubmed]
 
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