Involvement of zebrafish Na+,K+ ATPase in myocardial cell junction maintenance.
Na(+),K(+) ATPase is an essential ion pump involved in regulating ionic concentrations within epithelial cells. The zebrafish heart and mind (had) mutation, which disrupts the alpha1B1 subunit of Na(+),K(+) ATPase, causes heart tube elongation defects and other developmental abnormalities that are reminiscent of several epithelial cell polarity mutants, including nagie oko ( nok). We demonstrate genetic interactions between had and nok in maintaining Zonula occludens-1 (ZO-1)-positive junction belts within myocardial cells. Functional tests and pharmacological inhibition experiments demonstrate that Na(+),K(+) ATPase activity is positively regulated via an N-terminal phosphorylation site that is necessary for correct heart morphogenesis to occur, and that maintenance of ZO-1 junction belts requires ion pump activity. These findings suggest that the correct ionic balance of myocardial cells is essential for the maintenance of epithelial integrity during heart morphogenesis.[1]References
- Involvement of zebrafish Na+,K+ ATPase in myocardial cell junction maintenance. Cibrián-Uhalte, E., Langenbacher, A., Shu, X., Chen, J.N., Abdelilah-Seyfried, S. J. Cell Biol. (2007) [Pubmed]
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