Disease relevance of Zebrafish

• We showed that embryos with reduced sonic hedgehog (ref. 9) signalling and reduced tiggy-winkle hedgehog (ref. 10) function exhibit partial cyclopia and other specific midline abnormalities, providing a zebrafish genetic model for the common human disorder holoprosencephaly [1].
• To determine if a retroviral vector pseudotyped with the envelope glycoprotein of the vesicular stomatitis virus could infect zebrafish embryos, and in particular, the cells destined to become the germ line, a pseudotyped virus was injected into blastula-stage zebrafish embryos [2].
• This promoter binds to zebrafish Lef1 protein in vitro, and a nacre reporter construct is strongly repressed by dominant-negative Tcf in melanoma cells [3].
• Nitrosomorpholine caused not only hepatic tumors in guppies and zebra fish but also intestinal adenocarcinomas and poorly differentiated connective-tissue lesions in the abdominal cavities of zebra fish [4].
• The zebrafish mutation gridlock (grl, affecting the gene hey2) disrupts aortic blood flow in a region and physiological manner akin to aortic coarctation in humans [5].

Psychiatry related information on Zebrafish

• Rhythmic motor activity evoked by NMDA in the spinal zebrafish larva [6].
• Characterization of the Huntington's disease (HD) gene homologue in the zebrafish Danio rerio [7].
• This indicates that the zebrafish gene described here is a genuine member of the Id family, and suggests that it may serve a function similar to that of the Drosophila gene emc and mammalian Ids during development [8].
• Chlorpyrifos exposure of developing zebrafish: effects on survival and long-term effects on response latency and spatial discrimination [9].

High impact information on Zebrafish

• Furthermore, we identify genetic interactions between BBS genes and a PCP gene in both mouse (Ltap, also called Vangl2) and zebrafish (vangl2) [10].
• To elucidate the roles of eya4 in heart function, we studied zebrafish embryos injected with antisense morpholino oligonucleotides [11].
• In zebrafish, the structurally related switch molecule diversin ameliorates renal cysts caused by the depletion of inversin, implying that an inhibition of canonical Wnt signaling is required for normal renal development [12].
• We indentified and characterized a new gene, NIPBL, that is mutated in individuals with CdLS and determined its structure and the structures of mouse, rat and zebrafish homologs [13].
• Here we show that the gene u-boot (ubo), a mutation in which disrupts the induction of embryonic slow-twitch fibers, encodes the zebrafish homolog of Blimp-1, a SET domain-containing transcription factor that promotes the terminal differentiation of B lymphocytes in mammals [14].

Chemical compound and disease context of Zebrafish

• We found that embryos of the zebrafish Danio rerio can survive for 24 h in the absence of oxygen (anoxia, 0% O2) [15].
• Retinoic acid modifies development of the midbrain-hindbrain border and affects cranial ganglion formation in zebrafish embryos [16].
• We are utilizing zebrafish as a model system to determine whether there is an association between ethanol metabolism and ethanol-mediated developmental toxicity [17].
• Identification of zebrafish ARNT1 homologs: 2,3,7,8-tetrachlorodibenzo-p-dioxin toxicity in the developing zebrafish requires ARNT1 [18].
• The antineoplastic drug cisplatin, whose use in humans is limited by kidney toxicity, also causes typical histological changes and a decline in renal function in larval zebrafish [19].

Biological context of Zebrafish

• The zebrafish one-eyed pinhead (oep) mutation disrupts embryonic development, resulting in cyclopia and defects in endoderm, prechordal plate, and ventral neuroectoderm formation [20].
• Furthermore, we produce a PKD-like renal cystic phenotype and randomization of heart looping by knockdown of invs expression in zebrafish [21].
• Our results demonstrate that chordin function during gastrulation is important for the correct morphogenesis of the adult zebrafish skeleton [22].
• Wellcome Trust funds bid to unravel zebrafish genome [23].
• A homeobox gene essential for zebrafish notochord development [24].

Anatomical context of Zebrafish

• This mechanism also operates in zebrafish, indicating a conserved role for calcineurin/NFAT signaling in vertebrate heart valve morphogenesis [25].
• The cyclops mutation blocks specification of the floor plate of the zebrafish central nervous system [26].
• A zebrafish homologue of the chemokine receptor Cxcr4 is a germ-cell guidance receptor [27].
• We show that floating head is the zebrafish homologue of Xnot, a homeobox gene expressed in the amphibian organizer and notochord [24].
• Here we describe the zebrafish headless (hdl) mutant and show that its severe head defects are due to a mutation in T-cell factor-3 (Tcf3), a member of the Tcf/Lef family [28].

Associations of Zebrafish with chemical compounds

• Genetic screening identifies zebrafish mutants, such as fat free, that show normal digestive organ morphology but severely reduced phospholipid and cholesterol processing [29].
• Here, I outline the progress that has been made in answering these questions using the zebrafish pronephros as a simple, accessible model of nephron development [30].
• Pathfinding and synapse formation in a zebrafish mutant lacking functional acetylcholine receptors [31].
• Axial (HNF3beta) and retinoic acid receptors are regulators of the zebrafish sonic hedgehog promoter [32].
• In zebrafish, misexpression of Wnt-4, -5, and -11 stimulates calcium (Ca2+) release, defining the Wnt/Ca2+ class [33].

Gene context of Zebrafish

• We show that the zebrafish silent heart (sih) mutation affects the gene tnnt2 [34].
• Here we report target-selected inactivation of the dicer1 gene in zebrafish [35].
• The zebrafish mutant represents the first genetically 'accurate' animal model of HEP, and should be useful for studying the pathogenesis of UROD deficiency and evaluating gene therapy vectors [36].
• Functional knockdown of zebrafish tbx5 through the use of an antisense oligonucleotide resulted in a failure to initiate fin bud formation, leading to the complete loss of pectoral fins [37].
• Blocking Tsg function in zebrafish with morpholino oligonucleotides causes ventralization similar to that produced by chordin mutants [38].

Analytical, diagnostic and therapeutic context of Zebrafish

• Whole-mount in situ hybridizations on zebrafish embryos using a mixture of digoxigenin- and fluorescein-labelled probes [39].
• Here we describe the molecular cloning and primary structure of the zebrafish Wnt-1 gene (denoted wnt-1) [40].
• Northern blot analyses revealed that the zebrafish IGFBP-2 transcript is a 1.8-kb band expressed in many embryonic and adult tissues [41].
• Radiation hybrid panel mapping and analysis of a deletion mutant line (b240) indicate that a minimum of approximately 40 nitr genes are contiguous in the genome and span approximately 0.6 Mb near the top of zebrafish linkage group 7 [42].
• To this end, we have cloned 10 very divergent zebrafish odorant receptor molecules by PCR [43].

References