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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Analysis of several iridoid and indole precursors of terpenoid indole alkaloids with a single HPLC run.

An isocratic HPLC system is described which allows the separation of the iridoid and indole precursors of terpenoid indole alkaloids, which are present in a single crude extract. The system consists of a column of LiChrospher 60 RP select B 5 microm, 250 x 4 mm (Merck) with an eluent of 1% formic acid-acetonitrile-trichloroacetic acid (100:10:0.25, v:v:w) at a flow of 1.2 ml/min. In the suspension cultures of CATHARANTHUS ROSEUS secologanin and tryptophan were detected. In the cultures of TABERNAEMONTANA DIVARICATA loganin, tryptophan, and tryptamine accumulated.[1]


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