Disease relevance of Catharanthus

• T1, a cytokinin-inducible cytoplasmic periwinkle ( Catharanthus roseus ) protein, with significant sequence similarity to members of the Bet v 1 plant allergen family, was expressed in Escherichia coli [1].

High impact information on Catharanthus

• Here we report that auxin can generate transient changes in inositol-1,4,5-trisphosphate (Ins(1,4,5)P3) and inositol bisphosphate (InsP2) within minutes in Catharanthus roseus cells arrested in G1 [2].
• The gene for ORCA3, a jasmonate-responsive APETALA2 (AP2)-domain transcription factor from Catharanthus roseus, was isolated by transferred DNA activation tagging [3].
• These octadecanoid-derivative responsive Catharanthus AP2-domain (ORCA) proteins bind in a sequence-specific manner the JA- and elicitor-responsive element [4].
• The intracellular location of a cytochrome P-450-dependent monoterpene hydroxylase from the higher plant, Catharanthus roseus, has been investigated [5].
• Although genetic approaches are difficult for most plant species, promoter studies of single-pathway genes and T-DNA activation tagging are feasible alternative approaches for isolating transcription factors, as illustrated for terpenoid indole alkaloid biosynthesis in Catharanthus roseus [6].

Chemical compound and disease context of Catharanthus

• We describe the cloning, expression and characterization of a methionine synthase from the higher plant Catharanthus roseus. cDNAs were identified that encoded a protein of 85 kDa sharing 50% identify with the cobalamin-independent methionine synthase from Escherichia coli (MetE) and 41% identity with a partial sequence of a yeast homolog of MetE [7].

Biological context of Catharanthus

• Previously, we showed that the promoter of a Catharanthus roseus B-type cyclin, CYM, could direct M phase-specific transcription of a beta-glucuronidase reporter gene in synchronously dividing BY2 tobacco cells [8].
• A 2-oxoglutarate-dependent dioxygenase (EC 1.14.11.11) which catalyzes the hydroxylation at position 4 of the indole alkaloid, desacetoxyvindoline has been purified to near homogeneity from Catharanthus roseus [9].
• Novel type of receptor-like protein kinase from a higher plant (Catharanthus roseus). cDNA, gene, intramolecular autophosphorylation, and identification of a threonine important for auto- and substrate phosphorylation [10].
• Identification of a novel S-phase-specific gene during the cell cycle in synchronous cultures of Catharanthus roseus cells [11].
• Elicitor-responsive promoter regions in the tryptophan decarboxylase gene from Catharanthus roseus [12].

Anatomical context of Catharanthus

• Letter: Biosynthesis of the indole alkaloids. A cell-free system from Catharanthus roseus [13].
• Purification and properties of strictosidine synthetase (an enzyme condensing tryptamine and secologanin) from Catharanthus roseus cultured cells [14].
• Transport of inorganic orthophosphate (Pi) across the tonoplast membrane was studied using intact vacuoles isolated from suspension-cultured cells of Catharanthus roseus [15].
• A procedure for the quantitation of the delivery of liposome contents into Catharanthus roseus protoplasts has been developed [16].
• Effect of precursor feeding on alkaloid accumulation by a tryptophan decarboxylase over-expressing transgenic cell line T22 of Catharanthus roseus [17].

Associations of Catharanthus with chemical compounds

• The sequence of a cDNA clone that includes the complete coding region of tryptophan decarboxylase (EC 4.1.1.28, formerly EC 4.1.1.27) from periwinkle (Catharanthus roseus) is reported [18].
• The incorporation of [1-13C]- and [2,3,4,5-13C4]1-deoxy-D-xylulose into beta-carotene, lutein, phytol, and sitosterol in a cell culture of Catharanthus roseus was analyzed by NMR spectroscopy [19].
• Purification, characterization, and kinetic analysis of a 2-oxoglutarate-dependent dioxygenase involved in vindoline biosynthesis from Catharanthus roseus [9].
• Indole alkaloid biosynthesis in Catharanthus roseus: new enzyme activities and identification of cytochrome P450 CYP72A1 as secologanin synthase [20].
• Flavonoid hydroxylase from Catharanthus roseus: cDNA, heterologous expression, enzyme properties and cell-type specific expression in plants [21].

Gene context of Catharanthus

• The deduced amino acid sequence of bovine AADC shows 57% identity to drosophila AADC and 37% to plant Catharanthus roseus AADC [22].
• Cytochrome P450 2D6 (CYP2D6) inhibitory constituents of Catharanthus roseus [23].
• The most potent inhibitory activity against CYP3A4 (IC(50) value of 25 microg/ml) was found for the extract of Pi. nigrum leaf, while that of Catharanthus roseus showed the most potent inhibitory effect against CYP2D6 (IC(50) value of 11 microg/ml) [24].
• Isolation of cytochrome P-450 cDNA clones from the higher plant Catharanthus roseus by a PCR strategy [25].
• Expression of a feedback-resistant anthranilate synthase in Catharanthus roseus hairy roots provides evidence for tight regulation of terpenoid indole alkaloid levels [26].

Analytical, diagnostic and therapeutic context of Catharanthus

• Molecular cloning and analysis of strictosidine beta-D-glucosidase, an enzyme in terpenoid indole alkaloid biosynthesis in Catharanthus roseus [27].
• The application of the reverse phase high-performance liquid chromatography method (RP-HPLC) to the simultaneous separation of adenine nucleotides, in the presence of tonoplast vesicles isolated from Catharanthus roseus, showed results not necessarily correlated with the ATPase hypothesis [28].
• Direct fluorometry of phase-extracted tryptamine-based fast quantitative assay of L-tryptophan decarboxylase from Catharanthus roseus leaf [29].
• The enzyme acetylcoenzyme A:deacetylvindoline 4-O-acetyltransferase (EC 2.3.1.-) (DAT), which catalyzes the final step in vindoline biosynthesis in Catharanthus roseus, was purified 3300-fold using ammonium sulfate precipitation followed by gel filtration, anion exchange, hydroxyapatite, and affinity chromatographies [30].
• Two processes for the production of indole alkaloids 2 l surface-immobilized bioreactor cultures of Catharanthus roseus cells using Zenk's Alkaloid Production Medium (APM) were evaluated [31].

References