Nitric Oxide Synthase and PGE(2) Reciprocal Interactions in Rat Dental Pulp: Cholinoceptor Modulation.
In this study we determined the effect of cholinoceptor agonist pilocarpine on the stimulation of nitric oxide synthase (NOS) and on prostaglandin E(2) (PGE(2)) generation upon rat dental pulp. By reverse transcriptase/polymerase chain reaction (RT-PCR) we identified several products corresponding to m(1), m(2), m(3), and m(4) muscarinic acetylcholine receptors (mAChRs). The stimulation of M(1), M(2), M(3), and M(4) mAChRs by pilocarpine increases NOS activity and PGE(2) generation. There is a correlation (correlation coefficient = 0.05) between NOS activity and PGE(2) generation through the activation of phosphoinositide by phospholipase C (PLC), phospholipase A(2) (PLA(2)), and cyclooxygenase 1 ( COX-1). Exogenous PGE(2) restored NOS activity inhibited by indomenthacin (INDO), whereas nitric oxide (NO) donor restored PGE(2) generation inhibited by N(G)-methyl-L-arginine acetate salt (L-NMMA). These data indicate that both NO and PGE(2) interact with their own respective biosynthetic pathways modulating NOS and COX activities. Results could contribute to understanding the involvement of NO and PGE(2) in healthy dental pulp given that cellular signals through the parasympathetic system modulate the function of the dentin-pulp complex.[1]References
- Nitric Oxide Synthase and PGE(2) Reciprocal Interactions in Rat Dental Pulp: Cholinoceptor Modulation. Borda, E., Furlan, C., Orman, B., Reina, S., Sterin-Borda, L. Journal of endodontics (2007) [Pubmed]
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