The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Molecular and cellular studies of human immune responsiveness to the short ragweed allergen, Amb a V.

Specific immune responsiveness to the Amb a V allergen (from Ambrosia artemisiifolia, short ragweed pollen) is significantly associated with the Class II specificities, human leucocyte antigen (HLA)-DR2 and Dw2 determined by serological and MLR typing ("DR2.2"). Similarly, responsiveness to homologous Amb t V and Amb p V allergens is associated with DR2. 2. We examined the deoxyribonucleic acid (DNA) sequences of HLA-DRB1, DRB5, DQB1 and DQA1 genes associated with Amb a V responsiveness using a combination of polymerase chain reaction (PCR), dot-blot and DNA sequencing methodologies. Our focus was on the highly polymorphic regions within the second-exon gene segments that are believed to encode antigen (Ag)-binding portions of the respective Class II molecules. Analysis of three patients having unusual sequence combinations of HLA-D gene sequences implicate an HLA-DR molecule (either DR alpha beta I 2.2 or DR alpha beta V 2.2), rather than a DQ Class II molecule, as the major Amb a V immune response (Ir) gene product. Our studies suggest that this DR2.2 molecule is usually a necessary, and almost always a sufficient, requirement for high immunoglobulin E and G (IgE) and (IgG) antibody responsiveness in ragweed-allergic individuals. From an atopic DR2.2+ subject, we isolated three Amb a V-specific T-cell clones. Analysis revealed these clones to be DR-restricted, supporting the conclusion that the Amb a V-Ir gene is a DR and not a DQ molecule. The DR beta I polypeptide of DR2.2 and 2.12 was implicated in Ag presentation, since monoclonal antibody (MoAb) Hu30 (antibody specific for DR beta I) blocked T-cell proliferation.(ABSTRACT TRUNCATED AT 250 WORDS)[1]

References

  1. Molecular and cellular studies of human immune responsiveness to the short ragweed allergen, Amb a V. Marsh, D.G., Zwollo, P., Huang, S.K. The European respiratory journal. Supplement. (1991) [Pubmed]
 
WikiGenes - Universities