The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.
wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Thioredoxin interacting protein (TXNIP) was originally characterized as an endogenous inhibitor of thioredoxin, a key regulator in cellular redoxhomeostasis.TXNIP is also known to play important roles in tumor growth and metastasis, glucose and lipid metabolism.TXNIP expression is induced by various stress stimuli.However, it has been unclear how TXNIP is down-regulated.Here, we report that TXNIP undergoes proteasomal degradation in cells.We identify Itch as the E3 ubiquitin ligase for TXNIP.We demonstrate that Itchmediates polyubiquitination of TXNIP both in vitro and in vivo.Overexpression of Itch leads to TXNIP proteasomal degradation.Knockdown of Itch by small interfering RNA causes an accumulation of the steady-state level of TXNIP.We also show that the PPXY motifs of TXNIP and the WW domains of Itch mediate their interaction.Furthermore, the Itch-TXNIP interaction regulates intracellular reactive oxygen species levels and apoptosis.These findings establish a new mechanism for the negative regulation of TXNIP by Itch and shed new light on the regulation of cellular redoxhomeostasis.[1]
