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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Construction of aromatic dependent Shigella flexneri 2a live vaccine candidate strains: deletion mutations in the aroA and the aroD genes.

Live Shigella flexneri 2a vaccine candidate strains with deletion mutations in the aro genes were constructed. Tn10-generated auxotrophic mutations were transduced from Escherichia coli to S. flexneri 2a with bacteriophage P1CmCts. The tetracycline-sensitive derivatives of Tn10 mutants obtained were selected on Bochner's medium and checked by DNA-DNA hybridization using aroA and aroD gene specific probes. The vaccine candidate strains were tested to assess the efficacy of protection in guinea-pig conjunctival epithelia (Sereny test). The strains did not cause keratoconjunctivitis and exhibited significant protection in the challenge experiments. A candidate vaccine strain (delta aroD) showed 100% protection against 10(7) c.f.u. of wild type strain in the immunized guinea-pigs.[1]

References

  1. Construction of aromatic dependent Shigella flexneri 2a live vaccine candidate strains: deletion mutations in the aroA and the aroD genes. Verma, N.K., Lindberg, A.A. Vaccine (1991) [Pubmed]
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