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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Purification and heterogeneity of inorganic pyrophosphatase of pig scapula cartilage.

Inorganic pyrophosphatase (pyrophosphate phosphohydrolase; EC was purified from pig scapula cartilage by fractionation with N-cetylpyridinium chloride and (NH4)2SO4, followed by ion-exchange and gel-filtration chromatography. Enzyme preparations of high purity were obtained, with specific activities (100-400 units/mg) higher than those previously described for mammalian pyrophosphatases. The enzyme activity could be separated into several subfractions on ion-exchange columns.[1]


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