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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Serum alpha-fetoprotein subfractions in pregnant women identified by the modified method of lectin affinity crossed-line immunoelectrophoresis.

Using a modified method of lectin affinity crossed-line immunoelectrophoresis, we studied the alpha-fetoprotein ( AFP) subfractions in sera from 12 pregnant Japanese women, at 22 and 42 weeks of gestation. The method involves the first dimension electrophoresis in agarose gel containing concanavalin A (Con A) or lentil lectin ( LCH), the second dimension immunoelectrophoresis in agarose gel containing polyclonal antibody against AFP, reaction with peroxidase-conjugated Protein A and staining with 4-methoxy-1-naphthol. We found that type a of Con A or type C of LCH was the only subfraction present in maternal circulation at the second or third trimester. These AFP subfractions were assumed to be of fetal liver-origin. The minimum concentration which yielded an immunoprecipitation peak was approximately 100 ng/ml, being twenty times more sensitive than the conventional lectin affinity crossed-line immunoelectrophoresis.[1]

References

  1. Serum alpha-fetoprotein subfractions in pregnant women identified by the modified method of lectin affinity crossed-line immunoelectrophoresis. Ishiguro, T., Sakaguchi, H., Fukui, M., Sugitachi, I. Biological research in pregnancy and perinatology. (1985) [Pubmed]
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