Atrial natriuretic peptide and its messenger ribonucleic acid in overloaded and overload-released ventricles of rat.
To study the occurrence of atrial natriuretic peptide ( ANP) in overloaded ventricles, suprarenal aortic coarctation was performed on adult rats (n = 60) to induce overloaded left ventricle. In 36 rats, the overloads were released in 2 weeks. The hearts (6 rats in each group) were examined 2, 7, and 14 days after overload and 2, 7, 19, 47, and 77 days after overload release. Another group of 6 rats was coarctated for the second time for 1-6 days after 14 days of coarctation and 7 days of release. ANP immunoreactivity was examined by light and electron microscopic immunocytochemistry. ANP mRNA was studied by RNA-RNA tissue in situ hybridization. ANP immunoreactivity and ANP mRNA were found in the left ventricle 2 days after coarctation, and their amounts increased in proportion to the intraventricular pressure and the duration of coarctation. Two days after release, ANP and its mRNA began to decrease, but at 77 days ANP-containing granules still existed in the ventricular myocytes, whereas its mRNA became undetectable. The second coarctation triggered the release of the remaining ventricular ANP from the first overload and induced another cycle of increased ANP synthesis. Specific granules that do not contain ANP were found in overload-released ventricles, indicating the possible existence of other peptide hormones. These findings suggest that the extents of ventricular ANP gene expression and ANP synthesis and release are regulated by intraventricular pressure, and the occurrence of ANP in overloaded ventricles may not be a transient, immediately reversible phenomenon.[1]References
- Atrial natriuretic peptide and its messenger ribonucleic acid in overloaded and overload-released ventricles of rat. Gu, J., D'Andrea, M., Seethapathy, M. Endocrinology (1989) [Pubmed]
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