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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

Human and canine gene homologs of porcine brain natriuretic peptide.

A cDNA clone encoding porcine brain natriuretic peptide ( BNP) precursor was used to probe a canine genomic DNA library for homologous sequences. A unique clone was obtained (D1) which encoded a peptide homologous to porcine BNP. A 4.0 kB Hind III fragment of D1 containing the entire BNP precursor coding region was hybridized against blots of EcoRI digested human genomic DNA. A 7.0 kB hybridizing band was observed, which was subsequently subcloned and sequenced. This human clone, H1, also contained a sequence homologous to porcine BNP precursor. Transcription of the human BNP gene was confirmed by detection of BNP-specific sequences amplified from human atrial RNA by polymerase chain reaction. These results suggest that both BNP and ANP are present and function in a wide variety of mammalian species.[1]

References

  1. Human and canine gene homologs of porcine brain natriuretic peptide. Seilhamer, J.J., Arfsten, A., Miller, J.A., Lundquist, P., Scarborough, R.M., Lewicki, J.A., Porter, J.G. Biochem. Biophys. Res. Commun. (1989) [Pubmed]
 
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