Glutathione specifically labeled with isotopes.
A procedure for synthesis of glutathione selectivity labeled with isotopes is described. A strain of Escherichia coli enriched in its content of gamma-glutamylcysteine synthetase and glutathione synthetase by recombinant DNA techniques is immobilized in a carrageenan matrix and treated with toluene to render the cells more permeable to the substrates. The immobilized cell matrix is incubated with a mixture containing the appropriately labeled amino acid, the other amino acid constituents of glutathione, ATP, and acetylphosphate. The radiolabeled product is isolated by column chromatography.[1]References
- Glutathione specifically labeled with isotopes. Murata, K., Abbott, W.A., Bridges, R.J., Meister, A. Anal. Biochem. (1985) [Pubmed]
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