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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Helmy, S. et al.

Separation of endocytic from exocytic coated vesicles using a novel cholinesterase mediated density shift technique.

Coated vesicles isolated from rat liver perfused with diisopropylfluorophosphate (DFP) to inactivate endogenous cholinesterase contained newly synthesized secretory cholinesterase after a 30 min recovery. The cholinesterase is found in coated vesicles of presumed endocytic origin following DFP treatment and perfusion for 3 min with galactosylated cholinesterase, a ligand for the asialoglycoprotein receptor. Highly enriched populations of endocytic and exocytic coated vesicles can be separated by use of a novel cholinesterase mediated density shift technique. The two coated vesicle classes have very similar polypeptide compositions but differ significantly in the ratio of cholesterol to phospholipid.[1]

References

Separation of endocytic from exocytic coated vesicles using a novel cholinesterase mediated density shift technique. Helmy, S., Porter-Jordan, K., Dawidowicz, E.A., Pilch, P., Schwartz, A.L., Fine, R.E. Cell (1986) [Pubmed]

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