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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Molecular cloning of the sphingolipid activator protein-1 (SAP-1), the sulfatide sulfatase activator.

A cDNA coding for SAP-1 was isolated from a lambda gt11 human hepatoma expression library using polyclonal antibodies raised against human SAP-1. Three positive clones were isolated with inserts of approximately 0.3 Kb (S1.1), 2 Kb (S1.2) and 2.2 Kb (S-1.3). The latter 2 contained an internal EcoRI site. All three clones cross-hybridized with one another, indicating sequence homology. The nucleotide sequence of S-1.1 was determined. Colinearity was established between 19 amino acids obtained by sequencing the amino terminus of pure SAP-1 and 57 bp from the 5' end of S-1. 1. The open reading frame of S-1.1 coded for 67 amino acids. One glycosylation site was found 21 residues from the amino terminus, and no stop codons were found. S-1.1 codes for a mature polypeptide chain with a calculated molecular weight of 8955 daltons, corresponding to approximately 99% of mature SAP-1.[1]

References

  1. Molecular cloning of the sphingolipid activator protein-1 (SAP-1), the sulfatide sulfatase activator. Dewji, N., Wenger, D., Fujibayashi, S., Donoviel, M., Esch, F., Hill, F., O'Brien, J.S. Biochem. Biophys. Res. Commun. (1986) [Pubmed]
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