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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Plant glutamine synthetase complements a glnA mutation in Escherichia coli.

A glutamine synthetase gene from alfalfa (Medicago sativa) has been expressed in Escherichia coli after fusion of bacterial transcription and translation signals to a complete alfalfa glutamine synthetase coding sequence. Synthesis of the alfalfa glutamine synthetase enzyme in Escherichia coli was demonstrated by functional genetic complementation of a glutamine synthetase-deficient mutant and by immunoblotting analysis. These results should facilitate protein engineering and structure-function analysis of the plant enzyme.[1]

References

  1. Plant glutamine synthetase complements a glnA mutation in Escherichia coli. DasSarma, S., Tischer, E., Goodman, H.M. Science (1986) [Pubmed]
 
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