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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Cloning of cDNA coding for human tissue-type plasminogen activator and its expression in Escherichia coli.

cDNA clones of the mRNA coding for tissue-type plasminogen activator (t-PA) have been obtained and their nucleotide sequences compared to those reported previously. A gene coding for t-PA has been reconstructed and inserted into vectors for expression in prokaryotic cells. Relatively high levels of t-PA accumulated in inclusion bodies in Escherichia coli containing an optimized expression plasmid, but only a small proportion of the insoluble protein was recovered as active enzyme using a variety of solubilization procedures.[1]

References

  1. Cloning of cDNA coding for human tissue-type plasminogen activator and its expression in Escherichia coli. Harris, T.J., Patel, T., Marston, F.A., Little, S., Emtage, J.S., Opdenakker, G., Volckaert, G., Rombauts, W., Billiau, A., De Somer, P. Mol. Biol. Med. (1986) [Pubmed]
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