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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Quantitative studies o the activities of anti-human tonsil sera and anti-human spleen sera against B lymphocyte.

Anti-human B lymphocyte serum (AHBS) was obtained by absorbing rabbit anti-human tonsil serum (ATOS) or rabbit anti-human spleen serum (ASPS) with human red cells, liver, neutrophils, thymocytes and glutaraldehyde insolubilized human serum. Specificities of AHBS were checked by the cytotoxicity test, inhibition test of EAC-rosetting and inhibition of PWM responses. Raji cells of Burkitt's lymphoma originating from B-cells and acknowledged as non-immunoglobulin bearing on the cell surface were stained with AHBS according to the immunofluorescent technique. ATOS showed a higher cytotoxic titer against peripheral B-cells and inhibited EAC-rosetting stronger than ASPS did. On the other hand, macrophages in peripheral blood, Kupffer cells in the liver and certain cells of the thymus-medulla were stained with ASPA, but not with ATOS, even after intensively absorbing ASPS with thymocytes.[1]

References

  1. Quantitative studies o the activities of anti-human tonsil sera and anti-human spleen sera against B lymphocyte. Nishihira, T., Kunori, T., Tan, M., Tsutsumi, E., Kasai, M. Tohoku J. Exp. Med. (1977) [Pubmed]
 
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