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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Isolation and fusion studies on protoplasts from pollen tetrads.

Different enzymes were tested for isolation of intact protoplasts from pollen tetrads. About 80% isolation was achieved from pollen tetrads of Cajanus cajan and Zea mays and about 60% from Luffa cylindrica and Lycopersicon esculentum after 4 h of treatment with 5% cellulase. When these mononucleate protoplasts were incubated in presence of 0.05 M CaCl2 in 0.3 M glucose at pH 10.5, 70-80% fusion was achieved. Fusion was rare in sodium nitrate solutions.[1]

References

  1. Isolation and fusion studies on protoplasts from pollen tetrads. Deka, P.C., Mehar, A.K., Pathak, N.N., Sen, S.K. Experientia (1977) [Pubmed]
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