Regulatory mutants of the aroF-tyrA operon of Escherichia coli K-12.
The regulatory region of the aroF-tyrA operon was fused to the chloramphenicol acetyltransferase (cat) gene on a plasmid vector. Expression of the cat gene was subject to repression by tyrR+. This fusion was used to isolate regulatory mutants with increased expression of the cat gene in which repression by tyrR+ was affected. Nucleotide sequencing of these mutants has led to the identification of three sites involved in the repression of aroF by tyrR+. The existence of a functional promoter divergently transcribing from the aroF regulatory region was also demonstrated by using the cat fusion vector. The expression of this promoter is also regulated by tyrR+.[1]References
- Regulatory mutants of the aroF-tyrA operon of Escherichia coli K-12. Cobbett, C.S., Delbridge, M.L. J. Bacteriol. (1987) [Pubmed]
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