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Gene Review

aroF  -  3-deoxy-D-arabino-heptulosonate-7...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK2598, JW2582
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Disease relevance of aroF


High impact information on aroF


Chemical compound and disease context of aroF


Biological context of aroF

  • The tyr operon consists of promoter, operator, a short leader region without an attenuator, and two structural genes aroF and tyrA encoding the tyrosine-sensitive isoenzyme of 3-deoxy-D-arabinoheptulosonate-7-phosphate (DAHP) synthetase and chorismate mutase/prephenate dehydrogenase, respectively [5].
  • The mutations were found in two 18-base pair imperfect palindromes, called aroFo1 and aroFo2, which are located upstream of the aroF transcription start by 61 and 113 base pairs, respectively [1].
  • The gene order is pheA tyrA aroF in E. herbicola, with the same tail-to-tail arrangement of transcription known to exist in E. coli [6].
  • Tyrosine-mediated repression of aroF and tyrP was studied by inserting DNA sequences between the two adjacent TYR R boxes which, in each case, overlap the respective RNA polymerase binding sites of these genes [7].
  • An overexpression system (pCR105) for DAHP synthase (Tyr) was constructed by cloning the aroF gene at the NdeI site of the pET-22b(+) translation vector, a plasmid expression vector that contains the T7 lac promoter [8].

Associations of aroF with chemical compounds

  • In the present study, gene expression patterns of the shikimate producing strain W3110.shik1 (W3110 with aroL deletion and plasmid-overexpressed aroF) and the wild type strain W3110 grown under carbon- and phosphate-limited (carbon-rich) chemostat conditions (D=0.23h(-1)) were analyzed [9].

Other interactions of aroF


  1. Operator mutations of the Escherichia coli aroF gene. Garner, C.C., Herrmann, K.M. J. Biol. Chem. (1985) [Pubmed]
  2. Isolated operator binding and ligand response domains of the TyrR protein of Haemophilus influenzae associate to reconstitute functional repressor. Zhao, S., Somerville, R.L. J. Biol. Chem. (1999) [Pubmed]
  3. The nucleotide sequence of the aroF gene of Escherichia coli and the amino acid sequence of the encoded protein, the tyrosine-sensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase. Shultz, J., Hermodson, M.A., Garner, C.C., Herrmann, K.M. J. Biol. Chem. (1984) [Pubmed]
  4. Cloning of an aroF allele encoding a tyrosine-insensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase. Weaver, L.M., Herrmann, K.M. J. Bacteriol. (1990) [Pubmed]
  5. Nucleotide sequence and transcription of the phenylalanine and tyrosine operons of Escherichia coli K12. Hudson, G.S., Davidson, B.E. J. Mol. Biol. (1984) [Pubmed]
  6. The pheA/tyrA/aroF region from Erwinia herbicola: an emerging comparative basis for analysis of gene organization and regulation in enteric bacteria. Xia, T., Zhao, G., Jensen, R.A. J. Mol. Evol. (1993) [Pubmed]
  7. Importance of the position of TYR R boxes for repression and activation of the tyrP and aroF genes in Escherichia coli. Andrews, A.E., Dickson, B., Lawley, B., Cobbett, C., Pittard, A.J. J. Bacteriol. (1991) [Pubmed]
  8. Overexpression, purification, and characterization of tyrosine-sensitive 3-deoxy-D-arabino-heptulosonic acid 7-phosphate synthase from Escherichia coli. Ramilo, C.A., Evans, J.N. Protein Expr. Purif. (1997) [Pubmed]
  9. Transcriptome analysis of a shikimic acid producing strain of Escherichia coli W3110 grown under carbon- and phosphate-limited conditions. Johansson, L., Lid??n, G. J. Biotechnol. (2006) [Pubmed]
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