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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Cloning of the galactokinase gene (galK) from Streptomyces coelicolor A3(2).

Streptomyces coelicolor A3(2) and Streptomyces lividans 66 strains were shown to be sensitive to the galactose analogue 2-deoxy-D-galactose. Spontaneous resistant mutants were isolated that were Gal- and lacked the enzyme galactokinase. The galK gene ( structural gene for galactokinase) from S. coelicolor was cloned into S. lividans using the low copy number vector pIJ922. The resulting plasmid (pMT650), which contained a 14 kb insert, complemented gal mutations in both species. The presence of the galK gene on a 2.8 kb EcoRI fragment was confirmed by expressing it in Escherichia coli where it complemented a well characterized galK mutation.[1]

References

  1. Cloning of the galactokinase gene (galK) from Streptomyces coelicolor A3(2). Kendall, K., Ali-Dunkrah, U., Cullum, J. J. Gen. Microbiol. (1987) [Pubmed]
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