The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Peroxidative metabolism of a carcinogen, N-hydroxy-N-2-fluorenylacetamide, by rat uterus and mammary gland in vitro.

Lactoperoxidase-catalyzed metabolism of N-hydroxy-N-2-fluorenylacetamide (N-OH-2-FAA) may be via one-electron oxidation to nitroxyl free radical which dismutates to equimolar N-acetoxy-N-2-fluorenylacetamide and 2-nitrosofluorene (2-NOF) and/or a Br(-)-dependent oxidative cleavage to 2-NOF. Hence, the 2-NOF:N-acetoxy-N-2-fluorenylacetamide ratios reflect the relative contributions of the two peroxidative pathways to the metabolism of N-OH-2-FAA. Peroxidative activities of rat uterus (UT) and mammary gland (MG) were extracted with a cationic detergent, cetyltrimethylammonium bromide (Cetab). MG extracts had 1 to 5% the specific activity of UT extracts when assayed with guaiacol as hydrogen donor. At 0.004% Cetab, which in the incubation media corresponds to the approximate physiological levels of 0.1 mM Br(-), oxidation of N-OH-2-FAA by UT extracts yielded a product ratio indicative of both peroxidative pathways with Br(-)-dependent oxidation prevailing. At 0.4% Cetab, one-electron oxidation was negligible and Br(-)-dependent conversion of N-OH-2-FAA to 2-NOF was markedly enhanced. At both 0.004 and 0.4% Cetab, MG extracts yielded only 2-NOF, suggesting solely Br(-)-dependent oxidation. With equivalent guaiacol units of peroxidative activities, MG extracts produced much lower amounts of 2-NOF than did UT extracts. The low specific activities of MG extracts necessitated the use of larger amounts of protein, which might have interfered with peroxidative metabolism. At 0.004% Cetab, formation of 2-NOF by the Br(-)-dependent pathway was greater at pH 5.5 than at 7. 4. At acid pH, small amounts of 2-nitrofluorene were also formed by UT and MG extracts and could be attributed to further oxidation of 2-NOF. Peroxidative activities of the UT and MG extracts may be of granular leukocyte origin and their potential role in carcinogen activation and tumorigenesis is discussed.[1]


  1. Peroxidative metabolism of a carcinogen, N-hydroxy-N-2-fluorenylacetamide, by rat uterus and mammary gland in vitro. Malejka-Giganti, D., Ritter, C.L., Decker, R.W., Suilman, J.M. Cancer Res. (1986) [Pubmed]
WikiGenes - Universities