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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

An evaluation of the use of a pH indicator for the detection of beta-lactamase in enzyme immunoassay.

The use of a mixed pH indicator containing bromocresol purple and bromothymol blue was evaluated for the detection of beta-lactamase activity in enzyme immunoassays (EIA) based on this enzyme. The EIA was found to correlate well with a bioassay for antibodies to tetanus toxoid in sheep sera. Results could be read spectrophotometrically at 450 nm or visually as a colour change from dark blue to yellow. The mixed pH indicator was found to have a number of desirable features including good stability, ease of preparation and a sharp colour change which makes the system suitable for visual determination of titration end points.[1]

References

  1. An evaluation of the use of a pH indicator for the detection of beta-lactamase in enzyme immunoassay. Premier, R.R., Cox, J.C., Aitken, D.P., Healey, K. J. Immunol. Methods (1985) [Pubmed]
 
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