Disease relevance of BROMOMETHYLPHENOL

• All strains fermented glucose, sucrose, and fructose in purple broth base (Difco Laboratories) with bromocresol purple as indicator, although the atypical Pasteurella produced fermentation reactions that were barely perceptible [1].
• Problems associated with plasma albumin estimation in nephrotic syndrome using the bromocresol green method [2].
• Underestimation of albumin content by bromocresol green, induced by drug displacers and uremia [3].
• Initial (10 min) fluxes and tissue accumulations (Ac) were measured in the absence and presence of 1-5 mM BCG (bromocresol green; competitor organic anion) [4].

High impact information on BROMOMETHYLPHENOL

• We show here, however, that proton uptake, as indicated by bromocresol purple, and Schiff base deprotonation (380-nm absorption change) show different kinetics when the protein is solubilized in suitable detergents [5].
• Bromocresol purple was added to the samples to detect pH changes in the aqueous environment due to changes in the protonation state of rhodopsin [6].
• It can be differentiated from M. canis by the smaller size of its macroconidia, its failure to perforate hair in vitro, its poor growth and sporulation on bromocresol purple casein dextrose agar, and its incompatibility with Nannizzia otae, the telemorph of M. canis [7].
• In this study, the "pH memory" is explored by comparing the extent of protonation of sulfonephthalein probe molecules, bromophenol blue, bromocresol green, and chlorophenol red, in aqueous solution in the pH range of 3.4-6.0 and in the resulting freeze-dried amorphous matrix (lyophile) containing trehalose and sodium citrate buffer [8].
• We also developed conditions for detecting reversions by pH shift using bromocresol purple and by directly detecting the enzymatic activity of beta-lactamase using nitrocefin [9].

Biological context of BROMOMETHYLPHENOL

• Specific association of bromocresol purple anions with a magnesium complex of a phosphorylated intermediate during steady-state hydrolysis of ATP by the Mg2+ + Ca2+-dependent ATPase of sarcoplasmic reticulum [10].
• DESIGN: Participants with serum albumin values < or =3.5 g/dL (bromocresol green) or 3.2 g/dL (bromocresol purple) and a Mini Nutrition Assessment (MNA) Malnutrition score < or =23.5 were randomized to the supplement group (Nepro) or the nonsupplement group with intensive dietary counseling [11].
• Thus, IC50 of metronidazole was calculated at 25.5 +/- 2.3 mumol/l (n = 5) with the bromocresol purple assay and about 25 mumol/l after microscope observation [12].
• A series of 30 Saccharomyces cerevisiae aci(+) mutants (characterized as acidifying Ogur's glucose medium containing bromocresol purple) were isolated after EMS mutagenesis [13].

Anatomical context of BROMOMETHYLPHENOL

• The kinetics of the protonation changes and of the formation of the metarhodopsin II intermediate are measured in the presence of the pH indicator dye bromocresol purple after two consecutive flashes on the same suspension of right-side-out vesicles from cattle rod-outer-segment membranes [14].
• Serum albumin (bromocresol green) and CRP, age, dialysate to plasma (D/P) creatinine ratio, normalized protein catabolic rate (nPCR), daily urine and peritoneal protein losses, and extracellular fluid volume (Vecf) were measured in each patient [15].
• Dimer formation of bromocresol purple anions on the phosphorylated intermediate of sarcoplasmic reticulum [16].
• Serum free tri-iodothyronine (FT3), using two different radioimmunoassays, and serum albumin, using bromocresol green dye-binding method, were measured prospectively in 1374 adults investigated for thyroid disease [17].
• It is proposed that bromocresol green, and probably also amaranth, rapidly equilibrates between the hepatic and biliary compartments as a result of reabsorption from the biliary tree and that the rate-limiting factor in the biliary excretion of these dyes is the removal of dye from the biliary tree by bulk flow [18].

Associations of BROMOMETHYLPHENOL with other chemical compounds

• This latter aspect of the instrument is demonstrated by successfully determining the excited-state intensity decay kinetics for a dilute aqueous solution of rhodamine 6G dissolved in a high concentration of bromocresol green [19].
• The use of a mixed pH indicator containing bromocresol purple and bromothymol blue was evaluated for the detection of beta-lactamase activity in enzyme immunoassays (EIA) based on this enzyme [20].
• The bound enzyme activity can then be measured using a substrate solution containing urea and a pH indicator, most suitably bromocresol purple [21].
• Miso, a widely used Japanese fermented food was analysed for its lactic acid bacterial count on bromocresol purple agar [22].
• Two reagents, starch-iodine complex (SIC) and a mixed pH indicator, containing bromocresol purple and BTB (2:1) used earlier for the PNC-based ELISA, were compared with BTB for utilization in the PNC-based ELISA [23].

Gene context of BROMOMETHYLPHENOL

• Unlike the yeast orthologue, SAMC catalysed virtually only countertransport, exhibited a higher transport affinity for SAM and was strongly inhibited by tannic acid and Bromocresol Purple [24].
• METHODS: FSH, LH, SHBG by IRMA kits (DPC); E2, T, DHEAS, D4-A by RIA kits (Sorin Biomedica) after celite microcolumn chromatography; fT by coat-A-count kit (DPC); fE2 by equilibrium dialysis; serum albumin by bromocresol-green colorimetric methods [25].
• Bromocresol green, an inhibitor of prostaglandin transporter (PGT), strongly decreased [(3)H]PGE(1) transport across the BBB [26].
• The introduction of bromocresol purple for the determination of serum albumin on SMAC and ACA, and the standardization procedure [27].
• Moreover in quality control and calibration procedures, strong deviations from the recommended values are obtained when non-human sera are analyzed with the bromocresol purple method [28].

Analytical, diagnostic and therapeutic context of BROMOMETHYLPHENOL

• Measurement of serum albumin by capillary zone electrophoresis, bromocresol green, bromocresol purple, and immunoassay methods [29].
• Plasma concentrations of albumin and alpha(1)-acid glycoprotein (AGP) were determined by a bromocresol green method and a single immunodiffusion method, respectively [30].
• Serum albumin values from healthy cattle, sheep and horses determined by the immediate bromocresol green reaction and by agarose gel electrophoresis [31].
• The tissue albumin level was measured by nephelometry, and serum albumin level by Bromocresol green method [32].

References