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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Isolation and characterization of a genomic DDD mouse interleukin-3 gene.

A chromosomal DNA segment containing the entire gene for interleukin-3 (IL-3) was isolated from DDD mouse erythroleukemia cells, and its gene organization and nucleotide sequence were determined. Enhancer-like sequences in the second intron and G + C-rich sequence in the 5'-flanking region may play a role in the regulation of tissue-specific and inducible gene expression. Southern blot analyses revealed that constitutively IL-3-producing WEHI-3 cells have the rearranged IL-3 gene, and that genomic DNAs prepared from the adult and fetal mouse liver have the same organization of the IL-3 gene. No IL-3 gene transcript was detected in the mouse erythroleukemia cells by Northern blot analysis.[1]

References

  1. Isolation and characterization of a genomic DDD mouse interleukin-3 gene. Todokoro, K., Yamamoto, A., Amanuma, H., Ikawa, Y. Gene (1985) [Pubmed]
 
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