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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Transformation of human bronchial epithelial cells transfected by Harvey ras oncogene.

Transfection of normal human bronchial epithelial (NHBE) cells with a plasmid carrying the ras oncogene of Harvey murine sarcoma virus (v-Ha ras) changed the growth requirements, terminal differentiation, and tumorigenicity of the recipient cells. One of the cell lines isolated after transfection (TBE-1) was studied extensively and shown to contain v-Ha ras DNA. Total cellular RNA from TBE-1 cells hybridized to v-Ha ras structural gene fragment probes five to eight times more than RNA from parental NHBE cells. The TBE-1 cells expressed phosphorylated v-Ha ras polypeptide p21, showed a reduced requirement for growth-factor supplements, and became aneuploid as an early cellular response to v-Ha ras expression. As the transfectants acquire an indefinite life-span and anchorage independence they became transplantable tumor cells and showed many phenotypic changes suggesting a pleiotropic mechanism for the role of Ha ras in human carcinogenesis.[1]


  1. Transformation of human bronchial epithelial cells transfected by Harvey ras oncogene. Yoakum, G.H., Lechner, J.F., Gabrielson, E.W., Korba, B.E., Malan-Shibley, L., Willey, J.C., Valerio, M.G., Shamsuddin, A.M., Trump, B.F., Harris, C.C. Science (1985) [Pubmed]
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