Inhibition of synthesis of alpha-fetoprotein by glucocorticoids in cultured hepatoma cells.
alpha-Fetoprotein ( AFP) synthesis was studied in the presence and absence of glucocorticoids in rat hepatoma Mc-A-RH-7777 cells. Radioimmunoassay of media from cell cultures grown in the presence of glucocorticoid (dexamethasone or cortisol) showed a reduction in AFP, an increase in albumin, and no significant change in transferrin accumulation, as compared to controls. Labeling experiments with L-[35S]methionine indicated that in both cells and media of dexamethasone-treated cultures there was a 50--80% reduction in polypeptide precipitated by anti- AFP serum, as compared with controls; no change was seen in polypeptide precipitated by anti-transferrin serum. Pulse and pulse-chase experiments demonstrated that dexamethasone inhibited the synthesis of AFP but not its secretion. The half-time for secretion of AFP in the presence and absence of dexamethasone was 43 min.[1]References
- Inhibition of synthesis of alpha-fetoprotein by glucocorticoids in cultured hepatoma cells. Chou, J.Y., Mano, T., Feldman, M. J. Cell Biol. (1982) [Pubmed]
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