Thymidine kinase genes and the induction of anti-viral responses by interferon.
A mouse fibroblast cell-line deficient in thymidine kinase ( Ltk(-) aprt(-)) fails to show an anti-viral response when treated with interferon. After introduction of a viral tk gene into these cells the resultant clones showed normal responses to interferon. However, one such tk-containing clone (C6) spontaneously lost its ability to respond to interferon by inducing an antiviral state although it retained its ability to induce the enzyme oligo(2'-5' A)-synthetase. This sub-clone (6A) still expressed thymidine kinase activity but restriction endonuclease analysis indicated an alteration in the sequences flanking the exogenous viral tk gene. Our results suggest that a modification in the exogenous viral DNA sequences led to a loss of interferon sensitivity.[1]References
- Thymidine kinase genes and the induction of anti-viral responses by interferon. Mengheri, E., Esteban, M., Lewis, J.A. FEBS Lett. (1983) [Pubmed]
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