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Gene Review

LOC433762  -  hypothetical gene LOC433762

Mus musculus

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Disease relevance of LOC433762

  • Whereas unmethylated DNA demonstrated additional conformational features of active genes, such as DNAase I hypersensitivity and restriction endonuclease-sensitive segments, these markers were not present when methylated DNA was used for transfection [1].
  • UV damage could then be detected in a very sensitive quantitative assay such as that recently described using alkaline elution and an endonuclease preparation from Micrococcus luteus [2].
  • The DNA's from two of four methylcholanthrene-induced mouse fibrosarcomas contained transforming genes that were identical in their pattern of restriction endonuclease resistance to inactivation of biologic activity [3].
  • The patterns of the milk-transmitted (exogenous) mouse mammary tumor virus (MuMTV) DNA restriction endonuclease fragments in the nodule and tumor stages of BALB/cfC3H mouse mammary neoplasia were compared with the use of the Southern blot analysis [4].
  • Murine mammary tumor virus (MuMTV) provirus sequences in the DNA from early-occurring (average age 10 mo) and late occurring (age greater than 20 mo) tumors in BALB/cfC3H mice were analyzed by Eco RI restriction endonuclease mapping procedures [5].

High impact information on LOC433762

  • We evaluated these subjects for mutations in the gene for PPARgamma2 at or near a site of serine phosphorylation at position 114 that negatively regulates the transcriptional activity of the protein, using a polymerase-chain-reaction-based assay coupled with specific endonuclease digestion [6].
  • When 32 P-labeled DNA from each recombinant was hybridized to Southern blots of restriction endonuclease-digested DNA from different mouse cell lines, large differences were seen in the intensity of the resulting autoradiographic images, depending on the source of the genomic DNA [7].
  • Twenty-five cell generations later, the state of methylation of transferred DNA was examined by restriction endonuclease analysis and blot hybridization [8].
  • Based on restriction endonuclease digestions and the presence of human sequences in mouse transformants, we conclude that five of these human tumor cell lines contain a gene or genes capable of transforming mouse cells and that at least three different transforming genes are present in these five lines [9].
  • The genomic organization of the mouse dihydrofolate reductase gene has been determined by hybridization of specific cDNA sequences to restriction endonuclease-generated fragments of DNA from methotrexate-resistant S-180 cells [10].

Chemical compound and disease context of LOC433762


Biological context of LOC433762

  • The nucleotide sequence is partially corroborated by the sequence of fragments obtained previously from 32P-mRNA fingerprints and endonuclease IV digests of 32P-cDNA, and is in agreement with the amino acid sequence of the constant region, except for a rearrangement of four amino acids (between amino acid positions 163 and 166) [16].
  • In contrast, endonuclease-generated DNA fragments lacking this region on the map were unable to transform cells upon transfection [17].
  • Because some shared features of the structure of these two genes might be responsible for their coordinate expression and the elimination of their intervening sequences, we have compared their surrounding, coding and intervening sequences by restriction endonuclease analysis and by visualization of the heteroduplex structures formed between them [18].
  • Apoptosis. An endonuclease at last [19].
  • To ask whether the enhancers are differentially utilized by the endogenous gene in the three tissues in vivo, we examined their differential sensitivity to the endonuclease DNase I [20].

Anatomical context of LOC433762


Associations of LOC433762 with chemical compounds


Physical interactions of LOC433762


Enzymatic interactions of LOC433762

  • Third, Brca1(-/-) MEFs exhibited a 50-100-fold deficiency in microhomology-mediated end-joining activity of a defined chromosomal DNA double strand break introduced by a rare cutting endonuclease I-SceI [33].

Regulatory relationships of LOC433762

  • In this study, we analyzed the participation of the hepatic endonuclease deoxyribonuclease 1 (DNASE1) during APAP-induced hepatotoxicity by employing a Dnase1 knockout (KO) mouse model [34].
  • We found that forced over-expression of activated H-ras inhibited activation of the apoptotic endonuclease [35].
  • In an effort to establish the molecular basis of the expression of abnormal MHC molecules in these mice, we used MHC class I- and class II-specific cDNAs to probe endonuclease-digested genomic DNA from strains expressing lpr to look for restriction fragment length polymorphisms (RFLPs) [36].
  • Apoptotic endonuclease is a key enzyme that mediates regulated DNA fragmentation and chromatin condensation in response to apoptotic signals such as the Fas ligand, ionizing radiation, and anticancer agents [37].
  • These results demonstrate that NUC70 is an endogenous cytoplasmic endonuclease that is activated during apoptosis in a caspase-dependent mechanism [38].

Other interactions of LOC433762

  • Cloned myosin heavy chain DNA probes from rat and human were hybridized to restriction endonuclease digests of genomic DNA from somatic cell hybrids and their parental cells [39].
  • The Ercc1-Xpf heterodimer, a highly conserved structure-specific endonuclease, functions in multiple DNA repair pathways that are pivotal for maintaining genome stability, including nucleotide excision repair, interstrand crosslink repair and homologous recombination [24].
  • This molecular mechanism for the inhibition of CAD DNase by ICAD is similar to that proposed for colicin endonuclease and its inhibitor, immunity protein [40].
  • The constant region of Igl-1 differs between AKR/N and SJL/J with respect to a site for the restriction endonuclease KpnI [41].
  • On the other hand, restriction endonuclease sensitivity assay of chromatin showed that the promoter region, but not transcribed regions, of the transcribed Xist allele retained accessibility to nucleases [42].

Analytical, diagnostic and therapeutic context of LOC433762

  • A quantitative end-labeling assay showed that the level of m5C in the DNA of homozygous mutant cells was about one-third that of wild-type cells, and Southern blot analysis after cleavage of the DNA with a methylation-sensitive restriction endonuclease revealed substantial demethylation of endogenous retroviral DNA [43].
  • BER includes removal of the damaged base by a lesion-specific DNA glycosylase, strand scission by apurinic/apyrimidinic endonuclease, DNA resynthesis and ligation [44].
  • Site-directed mutagenesis demonstrates that the sequence surrounding the mapped endonuclease cleavage site is required for both iron-regulated mRNA turnover and generation of the truncated degradation intermediate [45].
  • Agarose gel electrophoresis of target cell DNA showed discrete multiples of an approximately 200-base-pair subunit, suggesting that fragmentation was the result of activation of a specific endonuclease [46].
  • The sequence organization of a recombinant clone, lambda . MTX-1, was analyzed by restriction endonuclease mapping, nuclease S1 mapping, and electron microscopy [47].


  1. DNA methylation affects the formation of active chromatin. Keshet, I., Lieman-Hurwitz, J., Cedar, H. Cell (1986) [Pubmed]
  2. Recombination of parent and daughter strand DNA after UV-irradiation in mammalian cells. Fornace, A.J. Nature (1983) [Pubmed]
  3. Frequent activation of c-kis as a transforming gene in fibrosarcomas induced by methylcholanthrene. Eva, A., Aaronson, S.A. Science (1983) [Pubmed]
  4. Alterations of acquired mouse mammary tumor virus DNA during mammary tumorigenesis in BALB/cfC3H mice. Cardiff, R.D., Morris, D.W., Young, L.J. J. Natl. Cancer Inst. (1983) [Pubmed]
  5. Detection of acquired provirus sequences in mammary tumors from low-expressor, low-risk mice. Altrock, B.W., Cardiff, R.D., Puma, J.P., Lund, J.K. J. Natl. Cancer Inst. (1982) [Pubmed]
  6. Obesity associated with a mutation in a genetic regulator of adipocyte differentiation. Ristow, M., Müller-Wieland, D., Pfeiffer, A., Krone, W., Kahn, C.R. N. Engl. J. Med. (1998) [Pubmed]
  7. Cloning of DNA from double minutes of Y1 mouse adrenocortical tumor cells: evidence for gene amplification. George, D.L., Powers, V.E. Cell (1981) [Pubmed]
  8. The somatic replication of DNA methylation. Wigler, M., Levy, D., Perucho, M. Cell (1981) [Pubmed]
  9. Human-tumor-derived cell lines contain common and different transforming genes. Perucho, M., Goldfarb, M., Shimizu, K., Lama, C., Fogh, J., Wigler, M. Cell (1981) [Pubmed]
  10. Structure and genomic organization of the mouse dihydrofolate reductase gene. Nunberg, J.H., Kaufman, R.J., Chang, A.C., Cohen, S.N., Schimke, R.T. Cell (1980) [Pubmed]
  11. Activation of a 15-kDa endonuclease in hypoxia/reoxygenation injury without morphologic features of apoptosis. Ueda, N., Walker, P.D., Hsu, S.M., Shah, S.V. Proc. Natl. Acad. Sci. U.S.A. (1995) [Pubmed]
  12. Chromosome healing in mouse embryonic stem cells. Sprung, C.N., Reynolds, G.E., Jasin, M., Murnane, J.P. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
  13. Photoreactivation of ultraviolet radiation-induced pyrimidine dimers in neonatal BALB/c mouse skin. Ananthaswamy, H.N., Fisher, M.S. Cancer Res. (1981) [Pubmed]
  14. Copper-zinc superoxide dismutase prevents the early decrease of apurinic/apyrimidinic endonuclease and subsequent DNA fragmentation after transient focal cerebral ischemia in mice. Fujimura, M., Morita-Fujimura, Y., Narasimhan, P., Copin, J.C., Kawase, M., Chan, P.H. Stroke (1999) [Pubmed]
  15. Overexpression of human copper/zinc-superoxide dismutase in transgenic animals attenuates the reduction of apurinic/apyrimidinic endonuclease expression in neurons after in vitro ischemia and after transient global cerebral ischemia. Narasimhan, P., Sugawara, T., Liu, J., Hayashi, T., Noshita, N., Chan, P.H. J. Neurochem. (2005) [Pubmed]
  16. Complete sequence of constant and 3' noncoding regions of an immunoglobulin mRNA using the dideoxynucleotide method of RNA sequencing. Hamlyn, P.H., Browniee, G.G., Cheng, C.C., Gait, M.J., Milstein, C. Cell (1978) [Pubmed]
  17. A defined subgenomic fragment of in vitro synthesized Moloney sarcoma virus DNA can induce cell transformation upon transfection. Andersson, P., Goldfarb, M.P., Weinberg, R.A. Cell (1979) [Pubmed]
  18. A comparison of two cloned mouse beta-globin genes and their surrounding and intervening sequences. Tiemeier, D.C., Tilghman, S.M., Polsky, F.I., Seidman, J.G., Leder, A., Edgell, M.H., Leder, P. Cell (1978) [Pubmed]
  19. Apoptosis. An endonuclease at last. Wyllie, A. Nature (1998) [Pubmed]
  20. Configuration of the alpha-fetoprotein regulatory domain during development. Godbout, R., Tilghman, S.M. Genes Dev. (1988) [Pubmed]
  21. Localisation of the G gamma-, A gamma-, delta- and beta-globin genes on the short arm of human chromosome 11. Jeffreys, A.J., Craig, I.W., Francke, U. Nature (1979) [Pubmed]
  22. Impaired thymic development in mouse embryos deficient in apoptotic DNA degradation. Kawane, K., Fukuyama, H., Yoshida, H., Nagase, H., Ohsawa, Y., Uchiyama, Y., Okada, K., Iida, T., Nagata, S. Nat. Immunol. (2003) [Pubmed]
  23. Defective DNA repair and increased genomic instability in Artemis-deficient murine cells. Rooney, S., Alt, F.W., Lombard, D., Whitlow, S., Eckersdorff, M., Fleming, J., Fugmann, S., Ferguson, D.O., Schatz, D.G., Sekiguchi, J. J. Exp. Med. (2003) [Pubmed]
  24. The structure-specific endonuclease Ercc1-Xpf is required for targeted gene replacement in embryonic stem cells. Niedernhofer, L.J., Essers, J., Weeda, G., Beverloo, B., de Wit, J., Muijtjens, M., Odijk, H., Hoeijmakers, J.H., Kanaar, R. EMBO J. (2001) [Pubmed]
  25. Transplantation of the human insulin gene into fertilized mouse eggs. Bürki, K., Ullrich, A. EMBO J. (1982) [Pubmed]
  26. Reduction in mitochondrial potential constitutes an early irreversible step of programmed lymphocyte death in vivo. Zamzami, N., Marchetti, P., Castedo, M., Zanin, C., Vayssière, J.L., Petit, P.X., Kroemer, G. J. Exp. Med. (1995) [Pubmed]
  27. Extracellular ATP as a trigger for apoptosis or programmed cell death. Zheng, L.M., Zychlinsky, A., Liu, C.C., Ojcius, D.M., Young, J.D. J. Cell Biol. (1991) [Pubmed]
  28. DNA cleavage in immunoglobulin somatic hypermutation depends on de novo protein synthesis but not on uracil DNA glycosylase. Nagaoka, H., Ito, S., Muramatsu, M., Nakata, M., Honjo, T. Proc. Natl. Acad. Sci. U.S.A. (2005) [Pubmed]
  29. Hydroxymethyluracil DNA glycosylase in mammalian cells. Hollstein, M.C., Brooks, P., Linn, S., Ames, B.N. Proc. Natl. Acad. Sci. U.S.A. (1984) [Pubmed]
  30. Allospecific and virus-specific cytolytic T lymphocytes are restricted to the N or C1 domain of H-2 antigens expressed on L cells after DNA-mediated gene transfer. Reiss, C.S., Evans, G.A., Margulies, D.H., Seidman, J.G., Burakoff, S.J. Proc. Natl. Acad. Sci. U.S.A. (1983) [Pubmed]
  31. Immunolocalization of a gonadotropin-releasing hormone receptor site in murine endometrium that mediates apoptosis. Murdoch, W.J. Cell Tissue Res. (1995) [Pubmed]
  32. Inducible expression and cytogenetic effects of the EcoRI restriction endonuclease in Chinese hamster ovary cells. Morgan, W.F., Fero, M.L., Land, M.C., Winegar, R.A. Mol. Cell. Biol. (1988) [Pubmed]
  33. BRCA1 facilitates microhomology-mediated end joining of DNA double strand breaks. Zhong, Q., Chen, C.F., Chen, P.L., Lee, W.H. J. Biol. Chem. (2002) [Pubmed]
  34. Deoxyribonuclease 1 aggravates acetaminophen-induced liver necrosis in male CD-1 mice. Napirei, M., Basnakian, A.G., Apostolov, E.O., Mannherz, H.G. Hepatology (2006) [Pubmed]
  35. Mutant H-ras over-expression inhibits a random apoptotic nuclease in myeloid leukemia cells. Moore, J., Boswell, S., Hoffman, R., Burgess, G., Hromas, R. Leuk. Res. (1993) [Pubmed]
  36. Southern blot analysis of major histocompatibility genes of lpr mice. Katagiri, T., Schepart, B., Croghan, T.W., Frelinger, J.A., Eisenberg, R.A., Cohen, P.L. Exp. Clin. Immunogenet. (1988) [Pubmed]
  37. Endonuclease activation and chromosomal DNA fragmentation during apoptosis in leukemia cells. Yoshida, A., Pommier, Y., Ueda, T. Int. J. Hematol. (2006) [Pubmed]
  38. Isolation and characterization of NUC70, a cytoplasmic, hematopoietic apoptotic endonuclease. Urbano, A., McCaffrey, R., Foss, F. J. Biol. Chem. (1998) [Pubmed]
  39. Multigene family for sarcomeric myosin heavy chain in mouse and human DNA: localization on a single chromosome. Leinwand, L.A., Fournier, R.E., Nadal-Ginard, B., Shows, T.B. Science (1983) [Pubmed]
  40. Enzymatic active site of caspase-activated DNase (CAD) and its inhibition by inhibitor of CAD. Sakahira, H., Takemura, Y., Nagata, S. Arch. Biochem. Biophys. (2001) [Pubmed]
  41. Linkage of the Igl-1 structural and regulatory genes to Akv-2 on chromosome 16. Epstein, R., Lehmann, K., Cohn, M., Buckler, C., Rowe, W., Davisson, M. Immunogenetics (1984) [Pubmed]
  42. Compact chromatin packaging of inactive X chromosome involves the actively transcribed Xist gene. Endo, Y., Watanabe, T., Mishima, Y., Yoshimura, A., Takagi, N., Kominami, R. Mamm. Genome (1999) [Pubmed]
  43. Targeted mutation of the DNA methyltransferase gene results in embryonic lethality. Li, E., Bestor, T.H., Jaenisch, R. Cell (1992) [Pubmed]
  44. The lyase activity of the DNA repair protein beta-polymerase protects from DNA-damage-induced cytotoxicity. Sobol, R.W., Prasad, R., Evenski, A., Baker, A., Yang, X.P., Horton, J.K., Wilson, S.H. Nature (2000) [Pubmed]
  45. Evidence that the pathway of transferrin receptor mRNA degradation involves an endonucleolytic cleavage within the 3' UTR and does not involve poly(A) tail shortening. Binder, R., Horowitz, J.A., Basilion, J.P., Koeller, D.M., Klausner, R.D., Harford, J.B. EMBO J. (1994) [Pubmed]
  46. Endogenous endonuclease-induced DNA fragmentation: an early event in cell-mediated cytolysis. Duke, R.C., Chervenak, R., Cohen, J.J. Proc. Natl. Acad. Sci. U.S.A. (1983) [Pubmed]
  47. Identification and molecular cloning of Moloney mouse sarcoma virus-specific sequences from uninfected mouse cells. Jones, M., Bosselman, R.A., van der Hoorn, F.A., Berns, A., Fan, H., Verma, I.M. Proc. Natl. Acad. Sci. U.S.A. (1980) [Pubmed]
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