Incorporation of alphafetoprotein by the MCF-7 human breast cancer cell line.
The intracellular uptake of alphafetoprotein ( AFP) and its fluorescein conjugates, by cultures of the MCF-7 human breast cancer cell line, has been demonstrated using an indirect immuno-peroxidase technique or by direct visualization under fluorescent lighting. The protein was localized in the cytoplasm. Ultrastructural autoradiographs of MCF-7 cells incubated with human 3H- AFP showed protein accumulation in several cytoplasmic organelles, particularly in lipid droplets. Nuclei were free of AFP. A significant species-specificity of AFP internalization was observed in comparative assays with human, mouse, pig and chicken AFP. The incorporation of the protein was prevented by incubation at 0 degrees C or by previous treatment of the cultures with 10nM sodium azide. Attention is paid to the reappearance in a human breast carcinoma cell line of a property associated during ontogenic development with ectodermal derivatives, including the epidermis.[1]References
- Incorporation of alphafetoprotein by the MCF-7 human breast cancer cell line. Uriel, J., Failly-Crepin, C., Villacampa, M.J., Pineiro, A., Geuskens, M. Tumour Biol. (1984) [Pubmed]
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